Front banner – Doering Lab

Scanning EM of cryptococci

Samira Kordestani — Tue, 11 Oct 2016 04:11:35 +0000

Wild type cryptococci imaged by scanning EM. Image by Felipe Santiago-Tirade. See Santiago-Tirado et al, 2016.

Fungi interacting with host phagocytes

Samira Kordestani — Tue, 11 Oct 2016 04:08:23 +0000

Cryptococcal cells (green) interacting with the human THP-1 phagocytes (nuclei, pink; cytosol, red). Image by Deepa Srikanta. See Srikanta et al, 2011.

Infected lung tissue

Samira Kordestani — Tue, 11 Oct 2016 04:04:11 +0000

Mucicarmine stain of a lung section from a mouse infected with C. neoformans, showing host inflammation, tissue destruction, and abundant fungi (walls stained maroon). See Gish et al, 2016.

Infected phagocytes on a model blood-brain barrier

Samira Kordestani — Tue, 11 Oct 2016 03:59:26 +0000

Human phagocytes (green) with engulfed cryptococci (red) interacting with a layer of brain endothelial cells (blue). Image by Felipe Santiago-Tirado. See Santiago-Tirado et al, 2017.

Negative stain of cryptococcal capsules

Samira Kordestani — Tue, 11 Oct 2016 03:53:13 +0000

Light micrograph of cryptococci induced to form capsule and then mixed with India ink. The space occupied by the capsule shows as a clear space between the gray background of the ink particles and the refractile edge of the cell.

Thin section EM of encapsulated cryptococci

Samira Kordestani — Tue, 11 Oct 2016 03:52:28 +0000

Thin section electron micrograph of wild type cryptococci after growth in capsule-inducing medium. Image by Aki Yoneda, colored by Wandy Beatty.

Close up of the edge of a cryptococcal cell

Samira Kordestani — Tue, 11 Oct 2016 02:08:32 +0000

Quick-freeze deep-etch image of the edge of a budding cryptococcal cell, showing a region of the plasma membrane (bottom left) bounded by the bilayered cell wall, with capsule fibers extending to the right from both the parent cell and a newly forming bud. Image by Tamara Doering and John Heuser.

Thin section EM of a budding cell

Samira Kordestani — Tue, 11 Oct 2016 01:39:37 +0000

Thin section of a budding C. neoformans that accumulates secretory vesicles because it has a sav1 mutation. Image by Aki Yoneda. See Yoneda et al, 2006.